Miscellaneous Immunology Report Templates

Andy Nguyen, MD


5/16/2022

 

1.CSF

 

CSF with restriction bands and corresponding bands in serum

There are two faint restriction bands in the gamma regions with corresponding bands in serum protein electrophoresis.

CSF protein electrophoresis results (see pattern description) are suggestive of a systemic monoclonal gammopathy and spillage of the monoclonal immunoglobulin into the CSF. Serum protein electrophoresis is suggested if clinically indicated. The CSF IgG index is elevated indicating that there is an increase in intracerebral IgG synthesis. There is no indication of increased permeability of the blood brain barrier based on the CSF/serum albumin ratio.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 84166-GC

 

CSF with increased permeability of the blood brain barrier

CSF protein electrophoresis did not reveal evidence of an oligoclonal process in the CNS. The CSF IgG index is within the reference range indicating that there is no elevation in intracerebral IgG synthesis. There is also evidence of increased permeability of the blood brain barrier based on the CSF/serum albumin ratio (in the absence of increase in RBC's in CSF).

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 84166-GC

 

CSF Oligoclonal bands

The CSF protein electrophoresis and immunofixation revealed presence of two faint oligoclonal bands in the CSF lane. The CSF IgG index is elevated indicating that there is an increase in intracerebral IgG synthesis. The CSF/serum albumin ratio is elevated that in the presence of a significant number of RBCs (281/mm3) is indicative of blood admixture to the CSF specimen.

The electronic medical record has been reviewed for relevant history.

These findings in a proper clinical setting are consistent with the presence on an oligoclonal process involving the CNS.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 84166-GC

 

CSF: no serum sample sent

CSF protein electrophoresis does not show an oligoclonal process in the CNS (see pattern description for limitations) but a concurrent serum sample was not submitted for comparison. For the same reason the CSF IgG index and the CSF/serum albumin ratio cannot be calculated.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 84166-GC

 

CSF: no serum sample sent; high RBC in CSF

CSF protein electrophoresis does not show an oligoclonal process in the CNS (see pattern description for limitations) but a concurrent serum sample was not submitted for comparison. For the same reason the CSF IgG index and the CSF/serum albumin ratio cannot be calculated. However, the intensity of staining in the CSF lane is most likely due to blood admixture in CSF specimen (the CSF RBC count is >58,000/mm3).

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 84166-GC

 

CSF:  a faint band is noted in the gamma region of the serum lane with no corresponding band in CSF.

The gel demonstrates appropriate resolution of the main protein bands. No oligoclonal bands are seen in the gamma region of the CSF lane. However, a faint band is noted in the gamma region of the serum lane.

The CSF protein electrophoresis does not reveal the presence of an oligoclonal process in the CNS. However, a band is noted in the gamma region of the control serum lane suggestive of a possible monoclonal gammopathy. Recommend serum and 24-hour immunofixation electrophoresis for confirmation.

The CSF IgG index is within the reference range indicating that there is no elevation in intracerebral IgG synthesis. There is also no evidence of increased permeability of the blood brain barrier based on the CSF/serum albumin ratio.

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 84166-GC

 

CSF: band in CSF with subsequent negative immunofixation

The gel demonstrates appropriate resolution of the main protein bands. The gamma region shows continuous distribution of proteins in the serum lane. There is a distinct single band in the CSF lane with a possible additional constriction with no corresponding bands in the serum lane.

The intensity of staining of the main protein fractions in the CSF lane is stronger than usually seen and is comparable with the intensity of staining in the serum lane.

CSF protein electrophoresis did not reveal definitive evidence of an oligoclonal process in the CNS. However, in a proper clinical setting, the described findings (see pattern description) could be suggestive of an oligoclonal process. Additionally, the distinct band could be associated with a chronic inflammatory process or, less likely, with brain plasmacytoma. The CSF IgG index is within the reference range indicating that there is no elevation in intracerebral IgG synthesis. The CSF/serum albumin ratio is elevated. In the absence of RBCs in the CSF specimen (0/mm3), this is consistent with increased permeability of the blood brain barrier.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 84166-GC

Addendum (xx/xx/xx): further CSF protein immunofixation shows polyclonal IgG and kappa band. No evidence of monoclonal gammopathy is found. The findings are supportive of a chronic inflammatory process.

 

CSF:  one faint monoclonal band in the CSF lane without corresponding band in the serum lane

The CSF protein electrophoresis revealed presence of one faint monoclonal band in the CSF lane without corresponding band in the serum lane. The CSF IgG index is elevated indicating that there is an increase in intracerebral IgG synthesis. There is no indication of increased permeability of the blood brain barrier based on the CSF/serum albumin ratio. Considering these findings, an oligoclonal process involving the CNS cannot be ruled out. Recommend follow-up with repeated testing at a later time and clinical correlation.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 84166-GC, 86335-GC

 

CSF-One monoclonal band

The gel demonstrates appropriate resolution of the main protein bands.  One faint band is present in the gamma region in the CSF lane that is not seen in the serum lane.

The CSF protein electrophoresis revealed presence of a solitary faint band in the CSF lane which is not present in the serum lane. The CSF IgG index is mildly elevated indicating that there is a slight increase in intracerebral IgG synthesis. There is increase in the CSF/serum albumin ratio indicating increased permeability of the blood brain barrier. These findings do not fulfill the criteria for oligoclonal process in the CNS. Clinical correlation is suggested

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 84166-GC

 

++++++++++++

2.SPE

 

SPE with distortion in beta region

Total protein is within the reference range. Serum protein electrophoresis shows normal distribution of the main protein fractions except for an increase in beta fraction. A distortion (0.81 g/dL) in the beta region is identified. This requires further evaluation by serum and 24-hour urine immunofixation to rule out monoclonal gammopathy.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT: 84165-GC

 

SPE with distortion in gamma region, normal immunofixation

Serum capillary immunofixation demonstration an increase in gamma globulin fraction with a distortion of the gamma region. However, serum immunofixation electrophoresis is negative for momonoclonal gammopathy (see separate report). Recommend evaluation by 24 hr urine immunofixation if clinically indicated.

Otherwise, total protein is within the reference range. Serum protein electrophoresis shows normal albumin level. Prominent polyclonal hypergammaglobulinemia is present. This could be seen in chronic inflammation, chronic liver disease, in autoimmune and lymphoproliferative disorders.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE with distortion in gamma region

Total protein is within reference range. Serum protein electrophoresis demonstrates mild hypergammaglobulinemia with a minimal decrease in the albumin fraction and mild beta-gamma bridging. A distortion is seen in gamma region. A monoclonal gammopathy cannot be ruled out.  This requires further evaluation by serum and 24-hour urine immunofixation.

Hypergammaglobulinemia could be associated with chronic inflammation, chronic liver disease, autoimmune and lymphoproliferative disorders. Presence of beta-gamma bridging is usually seen in chronic liver disease/liver cirrhosis. Clinical correlation is required.

I have personally reviewed the test results and concur with the resident's interpretation. `

CPT 84165-GC

 

SPE with distortion in gamma region

Capillary electrophoresis demonstrates a mild distortion of the gamma zone. This requires further evaluation by serum and 24 hr urine immunofixation to rule out monoclonal gammopathy. As there is no distinct peak, quantification is not possible.

Total protein level is within the reference range. Serum protein electrophoresis shows normal distribution of the main protein fractions.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE with distortion in gamma region

Serum protein electrophoresis shows a distortion in the gamma region. The polyclonal gamma globulin background is not suppressed.  Recommend serum and urine immunofixation electrophoresis for further evaluation.

Total protein level is within the reference range.  Serum protein electrophoresis shows relatively reduced albumin level and alpha-1 globulin fraction. These findings are otherwise consistent with chronic liver disease, protein loss, and possible inflammation. 

Please submit a 24-hour urine specimen.

I have personally reviewed the test results and concur with the resident’s interpretation.

CPT: 84165 GC

 

SPE with restriction in gamma region

Total protein is within reference range. Serum protein electrophoresis shows a restriction (0.83 g/dL) in the gamma region suggestive of monoclonal gammopathy. Recommend serum and urine immunofixation electrophoresis for further evaluation. Please submit a 24-hour urine specimen.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE with monoclonal band, SIFE with monoclonal bands

Total protein is within the reference range. Serum protein electrophoresis shows normal distribution of the main protein fractions. A monoclonal band (0.33 g/dL) in the gamma region is identified, suggestive of a monoclonal gammopathy. A corresponding serum protein immunofixation shows IgG-kappa monoclonal gammopathy (see separate report).

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT: 84165-GC

 

SPE with distortion in gamma region

Capillary electrophoresis demonstrates a distortion of the gamma zone. As there is no distinct peak, quantification is not possible.  This requires further evaluation by serum and 24 hr urine immunofixation.

Total protein is mildly decreased. Serum protein electrophoresis shows mildly reduced albumin level with relative elevation of the alpha-1 and gamma globulin fractions. This electrophoretic pattern is otherwise consistent with mild protein loss.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE: IgM-Kappa with faint IgG-Lambda bands, 66 y/o M with CLL

Total protein is within normal limits. Serum protein electrophoresis shows a distinct monoclonal peak (1.74 g/dL) and a secondary minor peak (0.16 g/dL) in the gamma region consistent with a monoclonal gammopathy. Serum immunofixation electrophoresis revealed a predominant monoclonal band of IgM-kappa isotype with secondary IgG-lambda gammopathy (see separate report). Clinical correlation is recommended.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE: hypogammaglobulinemia in a 78 y/o male

Total protein and albumin levels are within the reference ranges. A mild decrease in the gamma globulin fraction is present. No monoclonal bands are identified. Considering the age of the patient, hypogammaglobulinemia could be an indication of a light chain disease. Recommend urine protein immunofixation electrophoresis to rule out presence of Bence Jones proteins. Other causes of hypogammaglobulinemia, including lymphoid malignancies, have to be considered. Clinical correlation is required.

Please submit a 24-hour urine specimen.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 84165 GC

 

SPE: marked hypogammaglobulinemia in a 51 y/o female with normal serum IFE

Total protein level is normal. Serum protein electrophoresis shows relatively elevated albumin level and markedly reduced gamma globulin fraction with a mild distortion of the gamma distribution curve. The concurrent serum immunofixation study shows polyclonal distribution of immunoglobulin. However, this requires further evaluation by 24 hr urine immunofixation to rule out the presence of a light chain disease. Please submit 24-hour urine for immunofixation studies.

Other causes of hypogammaglobulinemia, including lymphoid malignancies and/or drug effect, also need to be considered. Clinical correlation is required.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE: Bisalbuminemia 

Total protein level is mildly elevated. Serum protein electrophoresis shows essentially normal distribution of the main protein fractions except for two bands in the albumin portion. This is consistent with bisalbuminemia associated with no clinical significance. No monoclonal bands are detected. Serum protein electrophoresis shows no significant pathologic changes.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

SPE: A prominent Spike suggestive of monoclonal gammopathy

Total protein is mildly elevated. Serum protein electrophoresis shows a prominent spike in the gamma region suggestive of a monoclonal band. The polyclonal gamma globulin background is significantly suppressed.  Based on the current electrophoretic scan, the concentration of the spike is approximately 2.83g/dL. Recommend serum and urine immunofixation electrophoresis for further evaluation.

Please submit a 24-hour urine specimen

I have personally reviewed the test results and concur with the resident’s interpretation.

CPT: 84165 GC

 

SPE: Slight Distortion in Gamma Region, 14 y/o male

Capillary electrophoresis demonstrates a slight distortion of the gamma zone. This requires further evaluation by serum and 24-hour urine immunofixation, although a monoclonal gammopathy is unlikely in this age group.

Total protein level is within the reference range. Serum protein electrophoresis shows normal distribution of the other main protein fractions.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 84165-GC

 

++++++++++++

3. IFE

sIFE free light chain

Serum immunofixation electrophoresis results (see pattern description) are suggestive of a possible low level monoclonal gammopathy of free lambda isotype. The polyclonal gamma globulin background is not affected. Recommend urine immunofixation electrophoresis for further investigation.

Please submit a 24-hour urine specimen.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 86335-GC

 

sIFE: Switching of light chain in B cell lymphoproliferative disorders

The immunofixation electrophoresis results (see pattern description) are consistent with monoclonal gammopathy of IgG-lambda isotype. Low intensity of protein staining in other lanes indicates suppression of synthesis of polyclonal immunoglobulins.

Of note, the patient has history of chronic lymphocytic leukemia, undergoing chemotherapy treatment. The previous serum immunofixation electrophoresis on xx/xx/xx showed a monoclonal gammopathy of the IgG-kappa isotype (report and gel were reviewed). The current serum immunofixation electrophoresis shows a monoclonal gammopathy of the IgG-lambda isotype. In light of this discrepancy, a repeated serum protein immunofixation at a later date is suggested if clinically indicated. Switching of light chain in B cell lymphoproliferative disorders has been reported after chemotherapy.

Relevant medical information in the EMR was reviewed.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 86334-GC

 

sIFE: IgM-Kappa with faint IgG-Lambda bands, 66 y/o M with CLL

A prominent monoclonal band is present in the IgM lane with a corresponding band in the Kappa light chain lane. There are also three faint bands in the IgG lane with corresponding faint bands in the Lambda lane. Faint diffusely staining immunoreactivity is present in IgG, IgA

The immunofixation electrophoresis results (see pattern description) are consistent with a predominant monoclonal gammopathy of IgM-Kappa isotype. There is also presence of monoclonal gammopathy of IgG-lambda isotypes at much lower quantity. 

Relevant medical information in the EMR was reviewed.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 86334-GC

 

sIFE: possible free lambda light chain

A very faint band is identified in the lambda lane with no corresponding bands in any of the other lanes. Diffusely staining immunoreactivity is present in IgG, IgA, IgM, kappa and lambda lanes in a normal distribution.

Serum immunofixation electrophoresis results (see pattern description) are suggestive of the presence of free monoclonal lambda light chains at a very low level. Follow-up of patients with repeated serum protein immunofixation at a later date is suggested if clinically indicated.

I have personally reviewed the test results and concur with the resident's interpretation.   

 

CPT 86335-GC

 

sIFE-Biclonality

The immunofixation electrophoresis results (see pattern description) are consistent with bi-clonal gammopathy IgG-kappa (predominant) and IgG-lambda (faint) isotypes. Preservation of diffusely staining immunoreactivity indicates that the production of polyclonal immunoglobulins is not suppressed.

Relevant medical information in the EMR was reviewed.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 86334-GC

 

sIFE: Very light staining

Light staining present in IgG and IgM lanes and no staining immunoreactivity is present in the IgA, kappa, and lambda lanes.

Recommend quantitative measurement of the serum immunoglobulins to rule out (primary or secondary) immunodeficiency if clinically indicated.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 86334-GC

 

uIFE: Constriction in kappa and lambda lanes

Urine immunofixation electrophoresis revealed spillage of polyclonal immunoglobulins mainly of IgG and IgA isotypes. The constrictions in kappa and lambda lane may represent polyclonal light chains due to impaired tubular reabsorption.

Relevant medical information in the EMR was reviewed.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 86335-GC

 

+++++

4.Hgb

 

Normal Hemoglobin electrophoresis

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern.

The electronic medical record has been reviewed for relevant history. This patient has microcytic hypochromic anemia. Differential diagnosis includes iron deficiency and beta thalassemia. Iron studies are unavailable from the EMR. Please note, iron deficiency may falsely lower HbA2 levels, thus masking beta thalassemia trait.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Elevated HgF in pregnancy

No abnormal hemoglobins are detected.  A minimal amount of Hgb F is present. Mild increase in Hgb F has been previously reported in normal pregnancy (Ref).

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation.

Ref: M. Ibrahim et al - Pattern of HB F Level Rise During Normal Pregnancies - 2009, Hemoglobin, Vol. 33, No. 6: Pages 534-538

CPT 83020-GC

 

Elevated HgF

No abnormal hemoglobins are detected. Hemoglobin F is slightly elevated (1.3%). Slight increases in hemoglobin F concentration are non-specific and may be found in a variety of unrelated hematologic disorders, such as aplastic anemia, hereditary spherocytosis, and myeloproliferative disorders. Hb F is commonly increased to as much as 5% to 10% in normal pregnancy. Slight elevation in hemoglobin A2 may also occur in hyperthyroidism or when there is deficiency of vitamin B12 or folate. Clinical correlation is recommended.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Normal Hgb with low MCV

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern.

This patient has microcytic hypochromic anemia. Differential diagnosis includes iron deficiency and beta thalassemia. Iron studies are unavailable from the EMR. Please note, iron deficiency may falsely lower HbA2 levels, thus masking beta thalassemia trait.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb SS, s/p transfusion, small C peak from donor

Per EMR, the patient has a diagnosis of sickle cell disease (Hb S/S) and was transfused on xx/xx/xx. Hemoglobin electrophoresis results are consistent with sickle cell disease (Hgb SS) status post transfusion.  The current levels of Hb A is 67.7 %, Hb S is 21.6 %, Hb F is 6.9 %, and Hb A2 is 2.5 %.

There is a minor peak in the C region (1.3 %), also seen on previous hemoglobin electrophoresis, which may be derived from donor and is clinically insignificant.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's

interpretation.

CPT 83020-GC

 

Hgb mild increase in Hgb F

Hemoglobin electrophoresis shows slightly elevated level of hemoglobin F (1%) and slightly decreased level of hemoglobin A. No abnormal hemoglobins are detected. An elevated level of hemoglobin F might be associated with numerous pathologic conditions such as pregnancy, aplastic anemia, anemia of chronic disease, pernicious anemia, hematologic malignancies etc. Recommend follow the patient and repeat hemoglobin studies in 6-12 months.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC                   

 

Hgb normal

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern

The electronic medical record has been reviewed for relevant history

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb normal with microcytic hypochromic anemia

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern

The electronic medical record has been reviewed for relevant history.  This patient has microcytic hypochromic anemia. No iron panel is available for review. Please note, iron deficiency may falsely lower hemoglobin A2 levels, thus masking beta-thalassemia trait.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb normal with iron deficiency anemia

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern except decreased hemoglobin A2 level.

The electronic medical record has been reviewed for relevant history.  This patient has microcytic hypochromic anemia and iron deficiency. Please note, iron deficiency may falsely lower hemoglobin A2 levels, thus masking beta-thalassemia trait. Recommend repeat hemoglobin electrophoresis, if anemia persists after treatment of iron deficiency.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Sickle cell trait vs sickle cell disease s/p transfusion

Hemoglobin electrophoresis results are consistent with sickle cell trait (Hb AS), unless the patient has been recently transfused (as in sickle cell disease, s/p RBC transfusion). Clinical correlation is suggested.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC 

 

Hgb SS vs. S/HPFH vs. S/Beta(0)

Hemoglobin electrophoresis shows presence of 67.8% of hemoglobin S and 25.6% of hemoglobin F. A small fraction of hemoglobin A is also seen (2.9%).  This may be consistent with the following conditions: homozygous S disease (Hb SS) with hydroxyurea therapy and remote transfusion, or S/ HPFH (S/hereditary persistence of fetal hemoglobin) with remote transfusion. S/beta (0) - thalassemia hemoglobinopathy with remote transfusion also cannot be excluded. Transfusion history as well as hematological and clinical correlation are necessary for final diagnosis.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hb S/S with high Hgb F

The patient has a history of sickle cell disease (Hb S/S). The current level of Hb S is 78.3%, the level of Hb F is 18.8%, and the level of Hb A2 is 2.9%. Hemoglobin electrophoresis results are consistent with sickle cell disease. Significantly elevated level of hemoglobin F may be due to either hydroxyurea treatment or to association of Hb SS with a specific haplotype known to have high level of hemoglobin F (Arab-Indian, for example). Hemoglobin S/HPFH, non-deletional form of hereditary persistence of fetal hemoglobin, is another possibility.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb Electrophoresis: Alpha Thal Trait

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern.

However, this patient is noted to have microcytic hypochromic anemia with normal iron studies. These findings are most suggestive of alpha thalassemia trait.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb SS vs Hgb S/HPFH

The current level of Hb S is 73.3%, the level of Hb F is 24.7%, and the level of Hb A2 is 2.0%. Hemoglobin electrophoresis results are consistent with either

(a) sickle cell disease (Hgb SS) on hydroxyurea, or

(b) Hgb S/HPFH (heterozygous S/Hereditary persistent Fetal Hgb)

Clinical correlation is suggested. 

I have personally reviewed the test results and concur with the resident's

interpretation.

CPT 83020-GC

 

Hgb SS vs Hgb S/HPFH, 1 y/o

The current level of Hb S is 69.7%, the level of Hb F is 28%, and the level of Hb A2 is 2.3%. Hemoglobin electrophoresis results are consistent with either

(a) sickle cell disease (Hgb SS) with high HgF in this 1 y/o female

(b) Hgb S/HPFH (heterozygous S/Hereditary persistent Fetal Hgb)

Repeated testing in 3-6 months would yield more definitive diagnosis. In sickle cell disease, Hgb F will decrease as patient grows older. In Hgb S/HPFH, Hgb F will stay elevated. Clinical correlation is suggested. 

I have personally reviewed the test results and concur with the resident's

interpretation.

CPT 83020-GC

 

Possible Alpha Thal Trait

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern.

The patient is noted to have microcytic hypochromic red blood cells but with normal iron studies and a normal Hb A2 level. Findings are suggestive of alpha thalassemia trait. Clinical correlation is required.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb Electrophoresis: cannot r/o Beta Thal trait

No abnormal hemoglobins are detected. Normal hemoglobin electrophoresis pattern. Per electronic medical record, this patient’s MCV and MCH are low (72.3 fL and 22.7 pg. respectively), but no iron studies are available for review. Hb A2 levels may falsely decrease in case of iron deficiency, thus interfere with the diagnosis of a concurrent beta-thalassemia trait. If the patient has the condition, recommend to repeat hemoglobin studies six weeks after correcting the iron status.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 83020-GC

 

Hgb Electrophoresis: Hgb AS or SS s/p transfusion, elevated HgF with placental abruption (A 84%, F 2.8%, S 10.4%, A2 2.8%)

Hemoglobin electrophoresis results may indicate sickle cell trait (Hb AS) after transfusion or sickle cell disease (Hb SS) after transfusion. Per review of the EMR, the patient was transfused on 7/7/2015. Also, patient has had recent pregnancy with placental abruption which may cause elevation in Hgb F (2.8%). Recommend repeat hemoglobin studies 3-6 months after latest transfusion. Clinical correlation necessary.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC 

 

Hgb Electrophoresis: elevated HgF in pregnancy (A 95.6%, F 1.9%)

No abnormal hemoglobins are detected. A slightly decreased level of Hgb A with a mild increase in Hgb F is present. Mild increase in Hgb F has been previously reported in pregnancy (Ref).

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

Ref: M. Ibrahim et al- Pattern of HB level rise during normal pregnancies, Hemoglobin, 2009, vol. 33, No.6: pages 534-538

CPT 83020-GC

 

Hgb A2’

Hemoglobin electrophoresis shows 97.7% of hemoglobin A, 1.3% of hemoglobin A2. There is a 1.0% peak in zone 1 (by capillary electrophoresis method) coordinating with 0.9% concentration at retention time of 4.49 min (by High Performance Liquid Chromatography method). This is consistent with heterozygous hemoglobin A2 variant (hemoglobin A2'), a finding of little clinical significance. The summation of hemoglobin A2 and hemoglobin A2 ' is 2.3%, which is within the reference range. This is an essentially normal hemoglobin electrophoresis pattern.

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb: Hgb E disease

Hemoglobin electrophoresis results are consistent with homozygous hemoglobin E hemoglobinopathy.  Hgb E is at 91.2%. Elevation of hemoglobin F is usually seen in this condition, which is almost entirely restricted to individuals of Southeast Asian origin. It is known to be a benign condition, which might present as a thalassemia minor. Reproductive counseling is important since double heterozygotes (E/beta-thalassemia) could develop severe thalassemic disorder.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb: Hgb SS disease with 6.2% Hg A, no Hx of transfusion available

Hemoglobin electrophoresis results are consistent with sickle cell disease

(Hgb SS).  The current level of Hb S is 80.0%, the level of Hb F is 10.5%, the level of Hb A2 is 3.3% and Hb A level is 6.2%. The presence of Hb A is likely due to previous transfusions, although no recent transfusion history is not available in the EMR.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb: Hgb D trait or G trait

Hemoglobin electrophoresis demonstrates 79.9% hemoglobin A and 17.9 % of a variant hemoglobin. Position of this hemoglobin band on capillary electrophoresis (between zones 5 and 6), together with HPLC retention time at 4.2 minutes, are supportive of Hemoglobin D trait.  Hemoglobin G trait is also a possibility. These traits are clinically insignificant. Clinical and hematological correlation are recommended.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb: Normal s/p transfusion, small S peak from donor

Per EMR, the patient has a low Hgb of 4.4 was transfused with 5 units of RBCs.

The current levels of Hb A is 93.2 %, Hb A2 is 2.5 %. There is a minor peak in the S region (4.4 %), which may be derived from donor (Hgb S trait donor) and is clinically insignificant.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb: Alpha Thal trait is a possibility

No abnormal hemoglobins are detected; normal hemoglobin electrophoresis pattern.

This patient has mild microcytic hypochromic anemia. Differential diagnosis includes iron deficiency, anemia of chronic disease, and thalassemia trait. Iron studies are within normal limits (ruling out iron deficiency anemia, and anemia of chronic disease).  Beta thalassemia trait is also ruled out with normal Hgb A2.  If alpha thalassemia trait is a clinical consideration, recommend genetic studies to rule it out if clinically indicated. 

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb Normal Neonate (15 hrs):

Hemoglobin electrophoresis shows elevated level of hemoglobin F (73.0%). No abnormal hemoglobins are detected. An elevated level of hemoglobin F is seen at this age as a variant of normal.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC 

 

Hgb: atypical band at zone 12, fresh sample

Hemoglobin electrophoresis shows presence of 89.4% hemoglobin A, 2.5% hemoglobin A2 and a significant amount (5.3%) of an unidentified hemoglobin, possibly degraded hemoglobin A. This abnormal hemoglobin was present in the previous sample also. 

Dr. xxxx's office had been called on xx/xx/xx and requested to send the fresh blood sample to the ARUP laboratories for Hemoglobin Evaluation Reflexive Cascade test (Test code: 2005792) for further confirmation. This has not been done and the current sample is being sent to ARUP for further evaluation.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC 

 

Hgb: S Trait at 6weeks old (15.6% A, 71% F, 13% S, 0.4% A2)

Hemoglobin electrophoresis results are consistent with sickle cell trait (Hb AS) unless the patient has been transfused.  An elevated level of hemoglobin F could be seen at this age (6 weeks old) as normal. However, an increase in hemoglobin F might be associated with hereditary persistence of fetal hemoglobin if elevated after one year of age. Recommend follow the patient and repeat hemoglobin studies when patient is one year old if clinically indicated.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

 

Hb G-Philadelphia trait

Hemoglobin (Hb) electrophoresis results by capillary method reveals a peak in zone 6 quantified at 33.2%; The Hb identification by High Performance Liquid Chromatography (HPLC) shows a G peak eluting at 4.17 minutes with a minor G2 peak at 4.56 minutes consistent with Hb G-Philadelphia variant. 

Hemoglobin G Philadelphia is an alpha globin variant and the most common gene variant in African Americans, also rarely seen in Chinese or Italians. Hb G-Philadelphia trait, as seen in this patient, has no clinical of hematological effects.  Individuals should be reassured that there are no clinical problems.

The electronic medical record has been reviewed for relevant medical information. This patient does not have a history of blood transfusions.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Sickle cell disease (Hb SS), status post RBC transfusion vs. Hgb S/beta (+) thalassemia hemoglobinopathy

Hemoglobin electrophoresis revealed 76.5% of hemoglobin S, 12.2% of hemoglobin A and 7.7% of hemoglobin F. The level of hemoglobin A2 is 3.6%. Per electronic medical record, this patient has sickle cell disease recently admitted with acute pain crisis and his MCV, MCH, RBC, and Hgb are low (77.9 fL, 26.9 pg, 3.31/mm3, and 8.9 respectively).  Considering this information and the CBC results the most likely diagnosis is homozygous cannot be completely ruled out. Hematological and clinical correlation is necessary.

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hb SS on a chronic transfusion protocol vs. a sickle cell trait individual massively transfused vs. a person recently transfused with blood products containing Hgb S

Hemoglobin studies revealed presence of 9.7% of hemoglobin S. She was recently admitted for acute on chronic anemia (Hgb 5.6) and was transfused 2 units of pRBC's on 7/12/2016. She has no history of sickle cell disease per EMR.  This low level of hemoglobin S could be seen either (a) in a patient with sickle cell disease (Hb SS) on a chronic transfusion protocol, not the case in the patient, (b) in a sickle cell trait individual massively transfused for any other cause, or (c) in a person who was recently transfused with blood products containing Hgb S (such as donors with S trait who are eligible to donate blood). Clinical correlation is suggested.

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC 

 

Sickle cell disease (Hb SS) status post transfusion/exchange or on a chronic exchange

Hemoglobin electrophoresis shows presence of 2.9% of Hb S, 94.3% of Hb A, and 2.8% of Hb A2. Per EMR the patient has known history of sickle cell disease (Hb SS) s/p stroke who is on chronic RBC exchange treatment and has undergone RBC exchange with 8 units of PRBC on 7/13/16. This result is consistent with sickle cell disease (Hb SS) status post transfusion/exchange or on a chronic exchange regiment. This is the post RBC exchange sample.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

An unidentified hemoglobin, located between zone 6 and zone 7 in capillary electrophoresis. 

Hemoglobin electrophoresis shows presence of 79% hemoglobin A, 2.8% hemoglobin A2 and a significant amount (18.2%) of an unidentified hemoglobin, located between zone 6 and zone 7 in capillary electrophoresis.  HPLC study picks up only 1.1% Hgb F in this 6-month-old male who had abnormal newborn screen with unknown hemoglobin.

To further work up this case, please send a fresh blood sample to the ARUP laboratories for Hemoglobin Evaluation Reflexive Cascade test (Test code: 2005792) for further confirmation.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC 

 

Sickle cell trait (Hb AS).

Hemoglobin electrophoresis results show the possibility of sickle cell trait (Hb AS), or sickle cell disease (Hb SS) with recent transfusion. According to EMR the patient has never received PRBSs. Therefore, the Hemoglobin electrophoresis is more consistent with sickle cell trait (Hb AS). Clinical correlation is suggested.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

ADDENDUM to send for Hemoglobin Evaluation Reflexive Cascade test

We were contacted by patient's hematology attending (Dr. xxxx) who would like to rule out Hgb S variants (such as S-Antilles) that would need more advanced testing techniques for detection. We suggested to Dr. xxxx to send sample to ARUP with specific order:

"Please send to ARUP laboratories for Hemoglobin Evaluation Reflexive Cascade test (Test code: 2005792) to rule out Hgb S Antilles or other Hgb S variants"

The immunofixation electrophoresis results (see pattern description) shows a faint constriction in the Ig A heavy chain lane with a corresponding faint constriction in kappa light chain lane. Preservation of diffusely staining immunoreactivity indicates that the production of polyclonal immunoglobulins is not suppressed. Recommend repeat the serum immunofixation electrophoresis in 3-6 months to rule out a low-level IgA-kappa monoclonal gammopathy

Relevant medical information in the EMR was reviewed.

I have personally reviewed the test results and concur with the resident's interpretation.   

CPT 86334-GC

 

Hgb S/beta-(0) thalassemia on Hydrea vs Hgb S/HPFH

The patient has a history of sickle cell disease.  Current hemoglobin study results reveal

Hgb S of 64.3%, Hgb F of 30.1%, and Hgb A2 of 5.6%. The CBC shows MCV 69.4 fL, MCH 22.8 pg, RBC 5.43. Considering these results, Hgb S/beta-(0) thalassemia is the most likely diagnosis. This is a sickling disorder. Knowledge of concurrent beta-thalassemia trait is important for future reproductive counseling. The high level of Hgb F is likely secondary to hydroxyurea treatment. If this patient is not on such treatment, heterozygous Hgb S and Hereditary Persistent Fetal Hemoglobin (Hgb S/HPFH) is a consideration. Clinical correlation is suggested.

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb SS with elevated HgF at 6 months old

Hemoglobin electrophoresis results are most consistent with sickle cell disease (Hgb SS).  The current level of Hb S is 63.9%, the level of Hb F is 34.3%, and the level of Hb A2 is 1.8%. The elevated HgF is associated with patient's age (6 months old). A repeated Hgb electrophoresis is suggested at 1 year of age for baseline.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Delta-beta thalassemia trait

No abnormal hemoglobins are detected. Hb F is high (10.9%) with normal level of other hemoglobins. Per electronic medical record, this patient’s MCV and MCH are low (66.2 fL and 22.0 pg. respectively). The findings are most supportive of delta-beta thalassemia trait.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT: 83020-GC

 

Sickle cell trait (Hb AS) at 2 week-old

Hemoglobin electrophoresis revealed 4.4 % of hemoglobin S, 5.1% of hemoglobin A and 90.5% of hemoglobin F.

Hemoglobin electrophoresis results are most consistent with sickle cell trait (Hb AS) unless the patient has been transfused.  An elevated level of hemoglobin F could be seen at this age (2 weeks old) as normal. However, an increase in hemoglobin F might be associated with hereditary persistence of fetal hemoglobin if elevated after one year of age. Recommend follow the patient and repeat hemoglobin studies when patient is one year old if clinically indicated.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC   

 

Hemoglobin SG

Hemoglobin studies (capillary electrophoresis and HPLC) detected two abnormal hemoglobins: HbS and HbG-Philadelphia. They revealed presence of 28.0% of hemoglobin S, 10.0% of hemoglobin G, 7.8% hemoglobin S/G, 0.8% hemoglobin G2, and 65.0% of hemoglobin A. According to the EMR, the patient had no recent RBC transfusions and had a history of anemia of unknown etiology. Considering this information, the findings are consistent with Hemoglobin SG.

HbG-Philadelphia is the most commonly occurring  hemoglobin caused by alpha-chain mutation. It is a stable, normally functioning hemoglobin not associated with any hematological abnormalities. Thus, hemoglobin SG, a combination of HbS and HbG  presents clinically as a sickle cell trait.

Medical records were reviewed for relevant clinical and laboratory information.

I have personally reviewed the test results and concur with the resident's Interpretation.

CPT 83020-GC   

 

5 week old female with HgF 99.8 %, Hgb A2 0.2%

No abnormal hemoglobins are detected. Hemoglobin F is elevated at 99.8%. No Hb A is present. These findings raise the possibility of several differential diagnoses:

1. Beta thalassemia major

2. Homozygous HPFH

3. Homozygous delta beta thalassemia

4. Double heterozygous state of beta thalassemia and delta beta thalassemia

Clinical correlation is required. Recommend repeat hemoglobin electrophoresis at 6 months of age for confirmation.

The electronic medical record has been reviewed for relevant medical information.

I have personally reviewed the test results and concur with the resident's interpretation

CPT: 83020-GC

 

Hgb H disease

Capillary hemoglobin electrophoresis shows Hgb H (zone 15) at 11.9%, Hgb A at 82.4%, Hgb F at 0.4%, Hgb A2 at 0.9%.  Additionally there is a small peak in zone 2 at 4.4% which may represent Hgb Constant Spring or Hgb C from RBC donors in previous RBC transfusion.

This patient is a 24 y/o Asian female who has microcytic hypochromic anemia. Patient had had a history of transfusion due to anemia. The overall findings are most consistent with Hgb H disease (alpha thalassemia major). Genetic testing is suggested if clinically indicated.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC

 

Hgb Lepore

Hemoglobin electrophoresis demonstrates 80.3% hemoglobin A, 8.5% hemoglobin F, and 8.7 % of a variant hemoglobin. Position of this hemoglobin band on capillary electrophoresis (in zone 6), together with HPLC retention time at 3.7 minutes, are supportive of Hemoglobin Lepore. Genetic testing for confirmation is suggested.

The electronic medical record has been reviewed for relevant history.

I have personally reviewed the test results and concur with the resident's interpretation.

CPT 83020-GC